NAD+
NAD+ (nicotinamide adenine dinucleotide, oxidized form; pyridine dinucleotide cofactor) · ≥98% (HPLC)
NAD+ (nicotinamide adenine dinucleotide) is a characterized dinucleotide cofactor reference material supplied for in-vitro studies of redox biochemistry and NAD-dependent enzyme systems. For research use only.
- Why researchers study it
- NAD+ is studied as a central redox-carrier cofactor and substrate/co-substrate in cellular-energy and NAD-metabolism research, where investigators examine its role in electron-transfer reactions and NAD-consuming enzyme pathways in cell-free and cultured-cell model systems.
- Mechanism themes in research
- Redox cofactor — reversible NAD+/NADH electron and hydride transfer in oxidoreductase reactionsCo-substrate for NAD-consuming enzymes (sirtuins, PARPs, CD38/ADP-ribosyl cyclases) studied in vitroPyridine-dinucleotide salvage and biosynthetic pathway intermediate in cellular-energy metabolism modelsADP-ribose / nicotinamide product chemistry probed in enzyme-mechanism studies
- What the evidence does not establish
- Published references are in-vitro or pre-clinical and describe research context only. They do not establish safety, efficacy, or any outcome in humans or animals, and Helix makes no such claims.
- Quality markers
- ≥98% (HPLC)MS identityLot COACold-chain
NAD+ (nicotinamide adenine dinucleotide, oxidized form) is a well-characterized pyridine dinucleotide cofactor supplied as a research-use-only reference material for in-vitro biochemistry. It comprises an adenine nucleotide and a nicotinamide nucleotide joined through a pyrophosphate bridge, and is widely used in the literature as a redox carrier and as a co-substrate for NAD-consuming enzyme classes studied in cell-free and cultured-cell systems.
Analytical identity
NAD+ is the oxidized form of nicotinamide adenine dinucleotide, free-acid form CAS 53-84-9, molecular formula C₂₁H₂₇N₇O₁₄P₂ with a free-acid molecular weight of approximately 663.43 g/mol (PubChem CID 5893; the protonated pyridinium cation is reported as C₂₁H₂₈N₇O₁₄P₂⁺). Catalog strength 500 mg, supplied as a lyophilized powder at ≥98% (HPLC). Identity and purity are intended to be confirmed by HPLC and spectroscopic methods on receipt.
Structure and mechanism themes
The molecule is a dinucleotide in which an adenosine 5'-monophosphate unit and a nicotinamide ribonucleotide unit are linked by a pyrophosphate bridge. In the research literature it functions as a reversible two-electron/hydride redox carrier (the NAD+/NADH couple) for oxidoreductase reactions, and as a cleavable co-substrate for NAD-consuming enzyme families — sirtuins, poly(ADP-ribose) polymerases, and CD38-type ADP-ribosyl cyclases — that release nicotinamide and transfer the ADP-ribose moiety. These mechanisms are characterized in cell-free enzyme assays and in cultured-cell metabolism models.
| Full name | NAD+ (nicotinamide adenine dinucleotide, oxidized form; pyridine dinucleotide cofactor) |
| Research theme | Mitochondrial & energy |
| CAS number | 53-84-9 |
| Molecular formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular weight | 663.43 g/mol |
| Purity | ≥98% (HPLC) |
| Physical form | Lyophilized powder |
| Storage | −20 °C, desiccated, protected from light |
Sequence
Reported research areas
- Cellular-energy and mitochondrial redox-metabolism research
- NAD-dependent enzyme (sirtuin / PARP / CD38) biochemistry
- Oxidoreductase enzyme assays and coupled-enzyme kinetics
- Analytical reference standard for NAD/NADH quantification methods
Credible sources
- PubChem — Nadide / NAD+ free acid (CID 5892)
- PubChem search — nicotinamide adenine dinucleotide
- PubMed — NAD+ metabolism literature
COA with every lot
Each NAD+ lot is characterized by reversed-phase HPLC for purity and by mass spectrometry for identity, then documented in a per-lot Certificate of Analysis. Unique lot numbers tie the vial back to its synthesis batch and analytical data.
- RP-HPLC purity result (≥98% (HPLC))
- Mass-spectrometry identity confirmation
- Appearance, molecular weight, and storage
- Lot number and testing status
Specimen Certificate of Analysis
| Product | NAD+ (reference) |
| Lot | HXR-2406-103 |
| Purity (RP-HPLC) | 98%+ |
| Identity (MS) | Confirmed · 663.43 g/mol |
| Appearance | Lyophilized powder |
| Storage | −20 °C, desiccated |
Representative document. Each lot has its own COA — these are quality documents, not medical claims.